Microdetermination of Phosphatases Employing Phenolphthalein Diphosphate as Substrate
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چکیده
منابع مشابه
An automated alkaline phosphatase assay with phenolphthalein monophosphate as substrate.
The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual ...
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A rapid and precise procedure for serum alkaline phosphatase employs phenolphthalein monophosphate as the substrate. This compound is readily hydrolyzed and directly provides a chromogen for colorimetric determination. The color produced is linear with respect to both enzyme concentration and incubation time and is stable. Reagent and serum blanks are negligible. Various factors which affect al...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1967
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/13.11.941